Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology. Follow step-by-step instructions that ensure experimental success Develop your own separation and analytical protocols for peptide and protein analysis.Trouble-Shooting 3.7.1. Column Runs Dry The single most common problem with gel filtration columns is running out of eluent, which causes the column to run dry and form cracks and voids. There is no easy fix when this happens, and theanbsp;...
|Title||:||HPLC of Peptides and Proteins|
|Publisher||:||Springer Science & Business Media - 2004|