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Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. Key Features * Presents up-to-date information * Provides extensive cross-referencing * Covers theory and practice in full detail * Written by leading authoritiesStep 1: Prepare assay tubes containing blockers, europium labeled primary antibody (clone 2F9) and serum samples or standards ... FIGURE 19.8 A schematic diagram of the measurement of serum estradiol using the idiometric assay, europium-labeled primary ... Estradiol standards (0a€“7000 pmol/liter) were prepared by using male sheep serum diluted 1:1 with assay buffer containing displacing agents.

Author:Eleftherios P. Diamandis, Theodore K. Christopoulos
Publisher:Academic Press - 1996-06-21


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