Intermediate Filament Cytoskeleton

Intermediate Filament Cytoskeleton

4.11 - 1251 ratings - Source

Intermediate filaments are a large family of proteins that are the cytoskeletal elements involved in a number of skin, liver, neuromuscular, cardiac, eye and hair diseases. Intermediate filament genes are regulated in a tissue-and cell type-specific manner and their polymerized protein products protects the cells and tissue they are part of against a variety of mechanical and nonmechanical stresses. This book provides a comprehensive resource of methodology essentials, describing a variety of essential tools and assays for studying intermediate filaments. The book provides user-friendly advice and protocols covering all aspects of intermediate filaments including protein isolation and structure, protein and gene regulation, relationship to disease and apoptosis, and associated proteins. Both mammalian and non-mammalian systems and animal models are covered, making this book a must-have for any investigator wishing to study IF genes or their protein products. * Covers intermediate filaments from crystallography, protein chemistry, cell and molecular biology, microrheology, gene regulation, to animal models and human disease * Practical and user-friendly with detailed qhow-to-protocols and qtricks of the tradeq * Includes detailed tables of useful reagents, vendors and web links... 768a€“770 overview, 766a€“767 plate assays, 772 transformation of yeast, 770a€“ 772 troubleshooting, 781a€“782 isoforms, ... histidine tag cleavage, 764a€“765 materials, 761a€“762 nickel affinity chromatography, 764 overview, 759a€“761, 780a€“ 781anbsp;...

Title:Intermediate Filament Cytoskeleton
Author:M. Bishr Omary
Publisher:Gulf Professional Publishing - 2004


You Must CONTINUE and create a free account to access unlimited downloads & streaming